Uses, Benefits, Cures, Side Effects, Nutrients in Crataeva Nurvala. List of various diseases cured by Crataeva Nurvala. How Crataeva Nurvala is effective for. Crataeva nurvala Buch. Ham. (Capparaceae) is a high-value medicinal tree that grows almost all over India, especially in the semiarid regions. Abstract. Crataeva nurvala Buch. Ham. (Capparaceae) is a high-value medicinal tree that grows almost all over India, especially in the semiarid.
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Antioxidant properties of Crataeva nurvala bark contains a variety of the bioactive phytochemical constituents in medicinal plants which include flavonoids, phenolic compounds, tannins, anthracene derivatives, and essential oils.
Components from Crataeva nurvala bark have been accounted to play an important role in scavenging free radicals generated by mutagens and carcinogens. Androgens are the key factors in either the initiation or progression of prostate cancer by inducing oxidative stress. In the present set of investigations, the antioxidative potential of Crataeva nurvala bark extract against androgen-mediated oxidative stress in male Wistar rats has been studied.
The prostate tissue was dissected out for biochemical analysis of lipid peroxidation and enzymic-antioxidants viz. Hence, the study reveals that constituents present in Crataeva nurvala bark impart protection against androgen-induced oxidative injury in prostate. Prostate cancer PCA continues to be the most frequently diagnosed neoplasm, and the second leading nurvxla of cancer-related mortality in men. Studies in culture system have shown that black tea extract and theaflavins are capable of inhibiting the growth of several human carcinoma cells including androgen-sensitive human prostate carcinoma cells LNCaP.
Varun/वरुण/Varuna/Crataeva nurvala/Three Leafed Caper
The source of hydrogen peroxide H 2 O 2 in tissues is mainly through superoxide dismutase SOD -medicated dismutation of O 2 – enerated in the tissues by endogenous enzyme system as well as by non-enzymatic pathways. ROS-associated oxidative damage is well documented in human PCA[ 91011 ] and down modulation of antioxidant enzymes in human prostate carcinoma cell lines viz.
It was authenticated by Dr. The phytochemical screening of Crataeva nurvala was performed as per procedure. The Wistar strain of male albino rats weighing between and g were used in the present study.
The animals were housed in large spacious cages and they were given food and water ad libitum during the course of the experiment. The experimental animals were divided crataega five groups. Each group consisted of six rats. After the experimental period, the animals were sacrificed under light chloroform anesthesia.
Crateva nurvala – Wikipedia
Crqtaeva was analyzed by the method of Okhawa. The tubes were then centrifuged; supernatants were separated and mixed with 1. Then, the mixture was heated for 30 minutes in boiling water.
The intensity of the pink-colored complex formed was measured at nm. The protein content of the tissue was determined by the method of Lowry[ 18 ] using bovine serum albumin as standard. SOD was analyzed as per the protocol of Kakkar. The control tube contained equal amount of buffer instead of sample.
The sample and its respective control were run together. At the end of the exposure time, 0. In brief, the assay mixture contained 0. The activity of GPx was estimated by the method of Rotruck[ 21 ] using sodium azide as substrate. The tubes were incubated for 0, 30, 60, and 90 seconds. The reaction was then terminated by the addition of 0. To determine the glutathione content, 2. The enzyme activity was measured at nm. GST was analyzed as per the protocol of Habig et al.
The increase in optical density of the enzyme was measured at nm. GR activity was measured by the protocol of Carlberg and Mannervik;[ 23 ] briefly, the assay mixture in a final volume of 3. The reaction was initiated with enzyme preparation. GSH was estimated by the method of Moran et al. The precipitate was removed by centrifugation. The absorbance was read within 2 minutes at nm against a reagent blank. A set of standards was also treated in the above manner.
Vitamin C was estimated by the method of Omaye et al. Absorbance was determined at nm. The statistical analysis was carried out by one-way analysis of variance using SPSS version 10 statistical analysis program. The phytochemical screening of the plant extract revealed the presence of major chemical constituents such as alkaloids, tannins, steroids, saponins, flavonoids, oils and fats, carbohydrates, amino acids, and proteins [ Table 1 ]. Preliminary phytochemical screening of the ethanolic extract of Crataeva nurvala.
Androgen is a known inducer of oxidative stress, and its intraperitoneal administration has been found to modulate the levels of antioxidant enzymes in male Wistar rats. The LPO activity in group 2 and group 5 animals were close to that of normal group 1 animals. Effect of Crataeva nurvala bark extract on lipid peroxidation in prostate tissue. The SOD levels in group 2 and group 5 animals were close to that of normal group 1 animals.
Effect of Crataeva nurvala bark extract on superoxide dismutase in prostate tissue.
GPx activity in group 2 and ctataeva 5 animals were close to that of normal crateava 1 animals. Effect of Crataeva nurvala bark extract on glutathione peroxidase in crataevx tissue. GST activity in group 2 and group 5 animals were close to that of normal group 1 animals as showed in Figure 5. Effect of Crataeva nurvala bark extract on glutathione-S-transferase in prostate tissue. Effect of Crataeva nurvala bark extract on glutathione reductase in prostate tissue.
Herbal medicines prescribed by tribal healers are either preparation based cratavea single plant part or a combination of several plant parts. They believe that combination of several plant parts cures diseases rapidly. Cancer chemoprevention by using antioxidant approaches has been suggested to offer a good potential in providing important fundamental benefits to public health, and is now considered by many clinicians and researchers as a key strategy for inhibiting, delaying, or even reversal of the process of carcinogenesis.
The phytochemical screening of the plant extract revealed the presence of major chemical constituents such as alkaloids, tannins, steroids, saponins, flavonoids, oils and fats, carbohydrates, amino acids, and proteins.
Some of their active principles including flavonoids are known to be used for the treatment of carcinogenic activity. Moreover, alkaloid-containing drug demonstrated anti-tumor activity. Several such compounds are known to possess potent antioxidant activity. In prostate tissue, LPO levels were elevated significantly in testosterone- and MNU-induced prostate cancer rats as compared to control rats.
Crataeva nurvala bark extract alone treated rats did cragaeva show any significant difference crataevx compared to normal rats. The comparison was done with standard drug finasteride-treated rats. Previous studies reported that finasteride prevents or delays the appearance of prostate cancer. Androgens are involved in the development of prostate cancer.
LPO is one of the main causes of oxidative damage initiated by ROS and it has been linked nirvala altered membrane structure and enzyme inactivation. It is initiated by the abstraction of a hydrogen atom from the side chain of PUFA in the membrane. There was no significant difference in Crataeva nurvala bark alone treated rats and their values were similar to control rats.
The major defense mechanism involves the antioxidant enzymes, including SOD, CAT, and GPx, which convert active oxygen rcataeva into non-toxic compounds. SOD is widely distributed in cells with high oxidative metabolism and has been proposed to protect against the deleterious effect of superoxide anion. SOD catalytically scavenges the superoxide radicals and thus renders cytoprotection against the free radical damage. Antioxidant activity and the inhibition of free radical generation are important in terms of protecting the prostate after treatment.
The most extensively nurvalx anti-oxidant enzyme in prostate cancer is GST.
Crataeva Nurvala Herb Uses, Benefits, Cures, Side Effects, Nutrients
In particular, expression of GST has been extensively studied in human prostatic tissues. Two thirds of normal basal prostatic epithelial cells immune stain intensely for GST, whereas acinar epithelia stain weakly for GST. GR is the most important biomolecule against chemically induced toxicity which can participate in the elimination of reactive intermediates by reduction of hydroperoxides in the presence of GPx.
It also functions as a free radical scavenger and in the repair of free radical caused biological damage. Glutathione being the most important biological molecule against chemically induced toxicity can participate in the elimination of reactive intermediates by reduction of hydro peroxides in the presence of GPx.
Glutathione also function as a free radical scavenger and in the repair of free radical-induced biological damage.
Crataeva Nurvala Herb Uses, Benefits, Cures, Side Effects, Nutrients
A high dose of vitamin C is reported to act as an oxidative agent. Membrane sulfhydryl groups play an important role in maintaining the structural integrity of membranes. Thus, the present study suggests that Crataeva nurvala bark can be used against test osterone- and MNU-induced oxidative damage in prostate of male albino rats.
The authors would like to express thanks to the Chairman and the Chancellor, the Advisor, the Vice chancellor, and the Registrar of Karpagam University, Coimbatore, India, for providing laboratory facilities and constant encouragement. National Center for Biotechnology InformationU. Journal List Pharmacogn Mag v.
Rathi3 Periasamy Meenakshi1 and Velliyur K.
Author information Article notes Copyright and License information Disclaimer. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3. This article has been cited by other articles in PMC. Reactive oxidative species, oxidative stress, testosterone, N-methyl-N-nitrosourea, antioxidant enzymes, Crataeva nurvala bark.
Preliminary phytochemical screening The phytochemical screening of Crataeva nurvala was performed as per procedure. Animals used The Wistar strain of male albino rats weighing between and g were used in the present study.
Induction of oxidative damage in prostate using carcinogen and hormone Step 1: Experimental design The experimental animals were divided into five groups. Biochemical estimations LPO was analyzed by the method of Okhawa. RESULTS The phytochemical screening of the plant extract revealed the presence of major chemical constituents such as alkaloids, tannins, steroids, saponins, flavonoids, oils and fats, carbohydrates, amino acids, and proteins [ Table 1 ].
Table 1 Preliminary phytochemical screening of the ethanolic extract of Crataeva nurvala.